LGC Biosearch Technologies launches Amp-Seq One: A revolutionary one-step targeted genotyping by sequencing workflow delivering speed and precision to enhance genomics in commercial breeding.

Set to become the fastest amplicon sequencing protocol on the market, Amp-Seq One offers reduced hands-on time and doubled throughput for customers.

31 October 2024 — LGC Biosearch Technologies™, a pioneer in agricultural genomics, today has announced the development of a revolutionary one-step amplicon sequencing workflow called Amp-Seq One that promises to be as fast as end-point genotyping with the total protocol turnaround time of just 120 minutes with only 50 minutes of PCR cycling.

This advancement makes Amp-Seq One the fastest amplicon sequencing protocol on the market while retaining compatibility with screening thousands of markers per reaction. Additionally, Amp-Seq One eliminates the requirement for liquid handling equipment to transfer material between reaction stages. The protocol halves the hands-on laboratory time, minimizes consumable use and lowers the risk of sample cross-contamination all while maximising workflow automation and doubling throughput.

Joris Parmentier, Associate Portfolio Director commented: “This ultra efficient approach uses room temperature stable reagents, cuts library preparation time to an industry leading 120 minutes and enables same-day progression from DNA sample to library creation and sequencing. Results to-date demonstrate the ability to target thousands of SNPs in a single reaction making it, a game changer in industrial genotyping for commercial breeding companies. The workflow can be further enhanced by using sbeadex™ Lightning that purifies DNA in under 5 minutes.”

Bharathi Anekella, Executive Vice-President LGC Diagnostics and Genomics Hub said: “Our on-going development of the agricultural genotyping portfolio reflects our continued commitment to investing in R&D. We aim to create tools in agriculture and Human Health Care that advance science and provide unique workable solutions for complex challenges. We take pride in our R&D history and our positive contributions to global society, and we will continue to strive to develop innovative solutions for our customers.”

Amp-Seq One is a customised panel kit that is created in collaboration with agriculture laboratories to meet their breeders specific genotyping goals and will be commercially available in the first half of 2025. This workflow has been designed to accelerate breeding programmes and adds to LGC Biosearch Technologies’ extensive portfolio of genotyping tools.

More information on how to evaluate Amp-Seq One

About LGC Biosearch Technologies
Biosearch Technologies is the comprehensive genomics portfolio from LGC, providing products and services for genomic analysis that support mission critical applications for global customers in agrigenomics. Our integrated tools and technologies enable our customers’ achievements whether through our revolutionary 5-minute purification technology (sbeadex™Lightning), oligonucleotides and reagents (KASP™, BHQplus™ probes, BHQ™ Probe Master Mix), and instrumentation (oKtopure™, SNPline, Nexar™, IntelliQube™). We also offer lab services for extraction, targeted genotyping by sequencing (Flex-Seq, Capture-Seq, nGBS/ddRAD) and genotyping workflows for use by customers’ laboratories (Amp-Seq, KASP). To learn more, visit: www.biosearchtech.com

Contacts

LGC Biosearch Technologies:
Business contact: Joris Parmentier, Product Management Associate Director, Applied; joris.parmentier@lgcgroup.com, +31 621 905 774

Media contact: Magda Bartoszewicz, Director, LGC Group Corporate Communications; magda.barto@lgcgroup.com, +44 7408 850 514